Anti-Ulcer Effect on Indomethacin-Induced Ulcerated Mice of Chromolaena odorata Leaf from Vietnam and its Secondary Metabolites

Chromolaena odorata (C. odorata) (L.) R. King & H. Robinson is a perennial flowering shrub with diverse habitats, including crops, grasslands, and roadsides. This plant has been widely used in Vietnamese folk medicine for gastric ulcer treatment. Hence, the present study aimed to evaluate acute toxicity anti-ulcer effect of ethanol crude extract C. leaves its fractions against indomethacin-induced model mice, investigate chemical constituents most active fraction. According vivo results, ethyl acetate residue highest activity significantly reduced lesions theexperimental mice an index 0.73 ± 0.39 percentage inhibition 26.92%. Thus, this fraction was chosen further investigation. Four pure compounds (1-4) were extracted isolated by using chromatographic methods. Based on nuclear magnetic resonance spectroscopy, melting temperature, mass spectrometry analysis, compared thepublished literature, their structures elucidated as 1-(4-hydroxy-3-methoxyphenyl)propane- 1,2-diol (1), kaempferol-7-O-α-L-rhamnopyranoside (2), naringenin-5,7-di-O-β-Dglucopyranoside (3), rubrosterone (4). To our best knowledge, all these first time from leaves. These findings contribute providing scientific evidence traditional use phytochemicals leaves.&nbsp